The flint assemblage of the Pottery Neolithic site of Tel Yosef was analyzed based on its stratigraphic position. Strata III and II yielded most of the flint artifacts, some of them were attributed to the Jericho IX culture, while the majority was dated exclusively to the Yarmukian culture. The Stratum I assemblage comprised objects dating to the Wadi Rabah phase. Nine tools, which predate the Pottery Neolithic period, possibly indicate human activity at the site prior to the Stratum III occupation.