Prehistoric research along the Cross-Israel Highway was undertaken from the earliest planning stages of the archaeological surveys along the intended route. During this survey, much data was accumulated concerning site function, economics, intra-site interaction etc. In the systematic survey and the subsequent excavations, 31 prehistoric sites were identified, dating from the Lower Paleolithic period to the Chalcolithic period, covering a time span of c. 500,000 years. This review presents a brief description of the surveyed and excavated sites for which there is available data, as well as a comparison with other sites from the same period.